A simple quantitative method using TLC-image analysis to determine fructooligosaccharides (FOS) in food samples
نویسندگان
چکیده
The thin-layer chromatography technique (TLC) is a simple and inexpensive analysis commonly used to identify qualitatively the presence of carbohydrates in food samples such as mono- di oligosaccharides particularly. TLC assay could be improved using image processing software for semiquantitative determination this type compound. In present work, TLC-image with Silica Gel 60 plates was 6 standards (glucose, fructose, sucrose, 1-kestose, nystose, fructofuranosylnystose). Subsequently, areas spots each compound were determined by digitizing conventional office scanner. Then, segmentation images carried out processing. calibration curves plotted Excel average pigmentations obtained pixels. study, also quantitatively determine honey, garlic, onion. Values coefficient (R2) greater than 0.97 all obtained. This useful detecting (monosaccharides, disaccharides, oligosaccharides) analytical assays without needing specialized equipment. it possible concentration garlic onion that showed prebiotic addition glucose, fructose. Key words:
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ژورنال
عنوان ژورنال: Turkish Journal of Chemistry
سال: 2022
ISSN: ['1300-0527', '1303-6130']
DOI: https://doi.org/10.55730/1300-0527.3436